平成30年度発足 新学術領域研究
発動分子科学

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研究成果 - C01-1

【原著論文】      【書籍/総説・解説】

原著論文
High-speed single-molecule imaging reveals signal transduction by induced transbilayer raft phases
I. Koyama-Honda, T. K. Fujiwara, R. S. Kasai, K. G.N. Suzuki, E. Kajikawa, H. Tsuboi, T. A. Tsunoyama, *A. Kusumi,
J. Cell Biol. , 219, e202006125. (2020), DOI:10.1083/jcb.202006125

Synergetic roles of Formyl Peptide Receptor 1 oligomerization in ligand-induced signal transduction
T. Nishiguchi, H. Yoshimura, R. S. Kasai, T. K. Fujiwara, *T. Ozawa,
ACS Chem. Biol. , 15, 2577-2587 (2020), DOI:10.1021/acschembio.0c00631

Domain architecture divergence leads to functional divergence in binding and catalytic domains of bacterial and fungal cellobiohydrolases
*A. Nakamura, D. Ishiwata, A. Visootsat, T. Uchiyama, K. Mizutani, S. Kaneko, T. Murata, K. Igarashi, *R. Iino,
J Biol Chem. , 295, 14606-14617 (2020), DOI:10.1074/jbc.RA120.014792 OA

Combined Approach to Engineer a Highly Active Mutant of Processive Chitinase Hydrolyzing Crystalline Chitin
A. Visootsat, A. Nakamura, T.W. Wang, and *R. Iino,
ACS Omega , 5, 26807–26816 (2020), DOI:10.1021/acsomega.0c03911

Single Cell Array Enclosed with a Photodegradable Hydrogel in Microwells for Image-Based Cell Classification and Selective Photorelease of Cells
S. Yamaguchi, R. Takagi, T. Hosogane, Y. Ohashi, Y. Sakai, S. Sakakihara, R. Iino, K. V. Tabata, H. Noji, and A. Okamoto,
ACS Appl. Bio Mater. , 3, 5887–5895 (2020), DOI:10.1021/acsabm.0c00583

Chemical-State-Dependent Free Energy Profile from Single-Molecule Trajectories of Biomolecular Motors:Application to Processive Chitinase
*K. Okazaki, A. Nakamura, R. Iino,
J. Phys. Chem. , 124, 6475–6487 (2020), DOI:10.1021/acs.jpcb.0c02698

Small stepping motion of processive dynein revealed by load-free high-speed single-particle tracking
J. Ando, T. Shima, R. Kanazawa, R. Shimo-Kon, A. Nakamura, M. Yamamoto, T. Kon, *R. Iino,
Sci. Rep. , 10 ,1080(2020), DOI:10.1038/s41598-020-58070-y

Single-molecule imaging analysis reveals the mechanism of a high-catalytic-activity mutant of chitinase A from Serratia marcescens
A. Visootsat, A. Nakamura, P. Vignon, H. Watanabe, T. Uchihashi, *R. Iino,
J. Biol. Chem. , 295, 1915-1925 (2020), DOI:10.1021/acsphotonics.9b00953

Multicolor High-Speed Tracking of Single Biomolecules with Silver, Gold, and Silver–Gold Alloy Nanoparticles
*J. Ando, A. Nakamura, M. Yamamoto, C. Song, K. Murata, *R. Iino,
ACS Photonics , 6, 2870-2883(2019), DOI:10.1021/acsphotonics.9b00953

Single-molecule analysis reveals rotational substeps and chemo-mechanical coupling scheme of Enterococcus hirae V1-ATPase
T. Iida, Y. Minagawa, H. Ueno, F. Kawai, T. Murata, *R. Iino,
J. Biol. Chem. , 294 ,17017-17030 (2019), DOI:10.1074/jbc.RA119.008947

Accurate high-throughput screening based on digital protein synthesis in a massively parallel femtoliter droplet array
*Y. Zhang, Y. Minagawa, H. Kizoe, K. Miyazaki, R. Iino, H. Ueno, K. Tabata, Y. Shimane, *H. Noji,Sci. Adv.,5, eaav8185 (2019), DOI: 10.1126/sciadv.aav8185

High-speed near-field fluorescence microscopy combined with high-speed atomic force microscopy for biological studies
T. Umakoshi, S. Fukuda, R. Iino, T. Uchihashi, *T. Ando,
Biochimica et Biophysica Acta (BBA) - General Subjects 1864(2019), DOI: 10.1016/j.bbagen.2019.03.011

Single-nanoparticle tracking with angstrom localization precision and microsecond time resolution
J. Ando, A. Nakamura, A. Visootsat, M. Yamamoto, C. Song, K. Murata, *R. Iino, Biophys. J., 115,2413-2427 (2018), DOI:10.1016/j.bpj.2018.11.016

Simultaneous Observation of Kinesin-Driven Microtubule Motility and Binding of Adenosine Triphosphate Using Linear Zero-Mode Waveguides
K. Fujimoto, Y. Morita, R. Iino, M. Tomishige, H. Shintaku, H. Kotera, *R. Yokokawa,ACS Nano , 12,11975-11985(2018),DOI:10.1021/acsnano.8b03803

Off-axis rotor in Enterococcus hirae V-ATPase visualized by Zernike phase plate single-particle cryo-electron microscopy
J. Tsunoda, C. Song, F. L. Imai, J. Takagi, H. Ueno, T. Murata, R. Iino, *K. Murata, Sci. Rep., 8, 15632 (2018), DOI: 10.1038/s41598-018-33977-9

Processive chitinase is Brownian monorail operated by fast catalysis after peeling rail from crystalline chitin
*A. Nakamura, K. Okazaki, T. Furuta, M. Sakurai, *R. Iino, Nat. Commun., 9, 3814(2018), DOI:10.1038/s41467-018-06362-3

Plasmid-Based One-Pot Saturation Mutagenesis and Robot-Based Automated Screening for Protein Engineering
F. Kawai, A. Nakamura, A. Visootsat, *R. Iino,
ACS Omega, 3, 7715-7726 (2018), DOI: 10.1021/acsomega.8b00663

書籍/総説・解説
・多色・高速1分子イメージング:金銀ナノ粒子による多色・高速生体1分子イメージング
*安藤潤, 中村彰彦, 山本真由子, ソンチホン, 村田和義, 飯野亮太, 光学 , 49, 249 (2020),

セラチア菌由来キチン加水分解酵素の運動機構
*中村彰彦, 岡崎圭一, 古田忠臣, 櫻井実, 飯野亮太, 応用糖質科学 , 6, 132-136 (2021),

銀, 金, 銀金合金ナノ粒子の光散乱を利用したマルチカラー生体1分子追跡
*安藤潤, *飯野亮太, フォトニクスニュース , 10, 89-95 (2020),

生きものが機械でもいいじゃない
飯野亮太, 生物物理 , 61, 77 (2021), DOI:10.2142/biophys.61.077

Crystalline chitin hydrolase is a burnt-bridge Brownian motor
A. Nakamura, K. Okazaki, T. Furuta, M. Sakurai, J. Ando, R. Iino,
Biophysics and Physicobiology , 17, 51-58 (2020), DOI:10.2142/biophysico.BSJ-2020004

Introduction: Molecular Motors
*R. Iino, *K. Kinbara, *Z. Bryant, Chem. Rev., 120, 1-4 (2020), DOI: 10.1021/acs.chemrev.9b00819

Defining raft domains in the plasma membrane
*A. Kusumi, T. K. Fujiwara, T. A. Tsunoyama, R. S. Kasai, A. A. Liu, K. M. Hirosawa, M. Kinoshita, N. Matsumori, N. Komura, H. Ando, K. G. N. Suzuki, Traffic, 21, 106-137 (2020), DOI: 10.1111/tra.12718

Visualization of Functional Structure and Kinetic Dynamics of Cellulases
*A. Nakamura, R. Iino,
Adv. Exp. Med. Biol., 1104 , 201-217 (2018), DOI: 10.1007/978-981-13-2158-0_10

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