平成30年度発足 新学術領域研究
発動分子科学

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研究成果 - C01

【原著論文】      【書籍/総説・解説】

原著論文
Dynamics of oligomer and amyloid fibril formation by yeast prion Sup35 observed by high-speed atomic force microscopy
H. Konno, T. Watanabe-Nakayama, T. Uchihashi, M. Okuda, L. Zhu, N. Kodera, Y. Kikuchi, T. Ando, *H. Taguchi, Proc. Natl. Acad. of Sci. USA, 117, 831-7836, DOI: 110.1073/pnas.1916452117

Dynamic behavior of an artificial protein needle contacting a membrane observed by high-speed atomic force microscopy
*T. Ueno, K. Niwase, D. Tsubokawa, K. Kikuchi, N. Takai, T. Furuta, R. Kawano, T. Uchihashi, Nanoscale,12, 8166-8173 (2020), DOI: 10.1039/D0NR01121E.

Plasmonic Manipulation of DNA using a Combination of Optical and Thermophoretic Forces:
Separation of Different-Sized DNA from Mixture Solution

T. Shoji, K. Itoh, J. Saitoh, N. Kitamura, T. Yoshii, K. Murakoshi, Y. Yamada, T. Yokoyama, H. Ishihara, *Y. Tsuboi, Sci. Rep., 10, 3349 (2020), DOI: 10.1038/s41598-020-60165-5

Single-molecule imaging analysis reveals the mechanism of a high-catalytic-activity mutant of chitinase A
from Serratia marcescens

A. Visootsat, A. Nakamura, P. Vignon, H. Watanabe, T. Uchihashi and *R. Iino, J. Biol. Chem., 295, 1915-1925 (2020), DOI: 10.1074/jbc.RA119.012078

High pressure inhibits signaling protein binding to the flagellar motor
and bacterial chemotaxis through enhanced hydration

H. Hata, Y. Nishihara, M. Nishiyama, Y. Sowa, I. Kawagishi, *A. Kitao, Sci. Rep., 10, 2351 (2020), DOI: 10.1038/s41598-020-59172-3

Crystal structures of the cell-division protein FtsZ from Klebsiella pneumoniae and Escherichia coli
T. Yoshizawa, J. Fujita, H. Terakado, M. Ozawa, N. Kuroda, S. Tanaka, R. Uehara, *H. Matsumura, Acta Crystallogr F Struct Biol Commun., 76, 86-93 (2020), DOI: 10.1107/S2053230X2000076X

Spectroscopic Signature of the Steric Strains
in an Escherichia coli RNase HI Cavity-Filling Destabilized Mutant Protein

C. Ota, H. Suzuki, S. Tanaka, *K. Takano, J Phys Chem B., 124, 91-100 (2020), DOI: 10.1021/acs.jpcb.9b09852

Supramolecular tholos-like architecture constituted by archaeal proteins without functional annotation
M. Yagi-Utsumi, A. Sikdar, C. Song, J. Park, R. Inoue, H. Watanabe, R. N. Burton-Smith, T. Kozai, T. Suzuki, A. Kodama, K. Ishii, H. Yagi, T. Satoh, S. Uchiyama, T. Uchihashi, K. Joo, J. Lee, M. Sugiyama, K. Murata and *K. Kato, Sci. Rep., 10, 1540 (2020), DOI: 10.1038/s41598-020-58371-2

Rad50 zinc hook functions as a constitutive dimerization module interchangeable with SMC hinge
H. Tatebe, C. T. Lim, H. Konno, K. Shiozaki, A. Shinohara, *T. Uchihashi, *A. Furukohri, Nat. Commun., 11
, 370 (2020), DOI: 10.1038/s41467-019-14025-0

Thermoresponsive Micellar Assembly Constructed from a Hexameric Hemoprotein Modified
with Poly(N-isopropylacrylamide) toward an Artificial Light-Harvesting System

S. Hirayama, *K. Oohora, *T. Uchihashi, *T. Hayashi, J. Am. Chem. Soc., 142, 1822-1831 (2020), DOI: 10.1021/jacs.9b10080

On-Membrane Dynamic Interplay between Anti-GM1 IgG Antibodies and Complement Component C1q
*S. Yanaka, R. Yogo#, H. Watanabe#, Y. Taniguchi, T. Satoh, N. Komura, H. Ando, H. Yagi, N. Yuki, *T. Uchihashi, *K. Kato, International Journal of Molecular Sciences, 21, 147 (2020), DOI: 10.3390/ijms21010147

Construction of a Hexameric Hemoprotein Sheet and Direct Observation of Dynamic Processes of Its Formation
*K. Oohora, S. Hirayama, *T. Uchihashi and *T. Hayashi, Chem. Lett., 49, 186-190 (2020), DOI: 10.1246/cl.190855,

Regular Assembly of Polymer Nanoparticles by Optical Trapping Enhanced with a Random Array of
Si Needles for Reconfigurable Photonic Crystals in Liquid

*I. Hanasaki, T. Shoji, *Y. Tsuboi, ACS Appl. Nano Mater., 2, 7637-7643 (2019), DOI: 10.1021/acsanm.9b01707

Crystal structure of heliorhodopsin
W. Shihoya, K. Inoue, M. Singh, M. Konno, S. Hososhima, K. Yamashita, K. Ikeda, A. Higuchi, T. Izume, S. Okazaki, M. Hashimoto, R. Mizutori, S. Tomida, Y. Yamauchi, R. Abe-Yoshizumi, K. Katayama, S. P. Tsunoda, M. Shibata, Y. Furutani, A. Pushkarev, O. Béjà, T. Uchihashi, *H. Kandori and *O. Nureki, Nature, 574, 132-136 (2019), DOI: 10.1038/s41586-019-1604-6

Nanotraffic Lights: Rayleigh Scattering Microspectroscopy of Optically Trapped Octahedral Gold Nanoparticles
*T. Shoji, M. Tamura, T. Kameyama, T. Iida, Y. Tsuboi, T. Torimoto, J. Phys. Chem. C, 37, 23096-23102 (2019), DOI: 10.1021/acs.jpcc.9b05077

The Fab portion of immunoglobulin G contributes to its binding to Fcγ receptor III
R. Yogo, Yuki Yamaguchi, H. Watanabe, H. Yagi, T. Satoh, M. Nakanishi, M. Onitsuka, T. Omasa, M. Shimada, T. Maruno, Tetsuo Torisu, S. Watanabe, D. Higo, T. Uchihashi, S. Yanaka, S. Uchiyama and *K. Kato,Sci. Rep. , 9,11957 (2019), DOI: 10.1038/s41598-019-48323-w

Protein Uptake into Individual Hydrogel Microspheres Visualized by High-Speed Atomic Force Microscopy
S. Matsui, K. Hosho, H. Minato, *T. Uchihashi, *D. Suzuki,
Chem. Commun.
,55,10064-10067 (2019),DOI: 10.1039/C9CC05116C

Dynamics of Inter-Molecular Interactions Between Single Aβ42 Oligomeric and Aggregate Species
by High-Speed Atomic Force Microscopy

L. Feng, H. Wantanabe, P. Molino, G. Wallace, S. Lam Phung, T. Uchihashi, *M. Higgins, Journal of Molecular Biology ,431, 2687-2699 (2019), DOI: 10.1016/j.jmb.2019.04.044

Investigation of Ag and Cu Filament Formation inside the Metal Sulfide Layer of an Atomic switch based on Point Contact Spectroscopy
A. Aiba, R. Koizumi, T. Tsuruoka, K. Terabe, K. Tsukagoshi, S. Kaneko, S. Fujii, T. Nishino, *M. Kiguchi,
ACS Appl. Mater. Inter.
, 11, 27178-27182 (2019), DOI:10.1021/acsami.9b05523

High-speed AFM reveals accelerated binding of agitoxin-2 to a K+ channel by induced fit
*A. Sumino, T. Sumikama, *T. Uchihashi, *S. Oiki,
Science Advances
, 5,eaax0495(2019), DOI:10.1021/acsami.9b05523

Identifying the Molecular Adsorption Site of a Single Molecule Junction
Through Combined Raman and Conductance Studies

S. Kaneko, E. Montes, S. Suzuki, S. Fujii, T. Nishino, K. Tsukagoshi, K. Ikeda, H. Kano, H. Nakamura, H. Vázquez, *M. Kiguchi, Chem. Sci., 10, 6261–6269 (2019),DOI: 10.1039/C9SC00701F

Effect of Bias Voltage on Single Molecule Junction Investigated by Surface Enhanced Raman Scattering
K. Yasuraoka, S. Kaneko, S. Fujii, T. Nishino, K. Tsukagoshi, G. Juhasz, *M. Kiguchi, J. Phys. Chem. C, 123, 15267−15272 (2019), DOI 10.1021/acs.jpcc.9b02286

Mutational and Combinatorial Control of Self-Assembling and Disassembling of Human Proteasome α Subunits
T. Sekiguchi, T. Satoh, E. Kurimoto, C. Son, T. Kozai, H. Watanabe, K. Ishii, H. Yagi, S. Yanaka S. Uchiyama, T. Uchihashi, K. Murata, *K. Kato
International Journal of Molecular Sciences
, 202608 (2019), DOI: 0.3390/ijms20092308

Non‐Thermoresponsive Decanano‐sized Domains in Thermoresponsive Hydrogel Microspheres
Revealed by Temperature‐Controlled High‐Speed Atomic Force Microscopy

Y. Nishizawa, S. Matsui, K. Urayama, T. Kureha, M. Shibayama, *T. Uchihashi and *D. Suzuki, Angew. Chem. Int. Ed. , 58,8809-8813 (2019),DOI:10.1002/anie.201903483

Activity-stability trade-off in random mutant proteins
R. Kurahashi, S. Tanaka, *K. Takano, J Biosci Bioeng. , 128, 405-409 (2019),
DOI: 10.1016/j.jbiosc.2019.03.017.

Construction of a Quadrangular Tetramer and a Cage-Like Hexamer
from Three-Helix Bundle-Linked Fusion Proteins

T. Miyamoto, Y. Hayashi, K. Yoshida, H. Watanabee, T. Uchihashi, K. Yonezawa, N. Shimizu, H. Kamikubo, *S. Hirota, ACS Synth. Biol.,81112-1120 (2019), DOI: 10.1021/acssynbio.9b00019

Hydrogel Microellipsoids that Form Robust String‐Like Assemblies at the Air/Water Interface
K. Honda, Y. Sazuka, K. Iizuka, S. Matsui, T. Uchihashi, T. Kureha, M. Shibayama, T. Watanabe, *D. Suzuki,
Angewandte Chemie International Edition
, 58, 7294-7298 (2019), DOI:10.1002/anie.201901611

書籍/総説・解説
Recent advances in bioimaging with high-speed atomic force microscopy
*T. Uchihashi, C. Ganser, Biophysical Reviews, 12, 363-369 (2020), DOI:10.1039/D0NR01121E

Diffracted X-ray tracking method for recording single-molecule protein motions
*H. Shimizu, Biochim Biophys Acta Gen Subj. , 1864, 129361 (2020), DOI: 10.1016/j.bbagen.2019.05.004.

高速原子間力顕微鏡による生体・合成高分子の動態イメージング
*内橋貴之, 高分子, 68, 564-568 (2019)

・"ナノ構造体を用いる光ピンセットの開発”, 東海林竜也, 分析化学, 68, 315-324 (2019), DOI: 10.2116/bunsekikagaku.68.315

・「図説 表面分析ハンドブック」, 日本表面真空学会 (Ed.) ,
“高速原子間力顕微鏡”, 内橋貴之, 23. 8, 朝倉書店, (2020), ISBN: 978-4-254-20170-3 C3050

・「膜タンパク質工学ハンドブック」, 津本浩平・浜窪隆雄 (Eds) ,
“高速原子間力顕微鏡によるタンパク質の構造ダイナミクス解析” , 内橋貴之, エヌ・ティー・エス, (2020), 第1編 第2章15, ISBN: 978-4-86043-537-0 C3045

・「自己修復材料、自己組織化、形状記憶材料の開発と応用事例」,
“高速原子間力顕微鏡による分子の自己組織化過程のリアルタイムでの観察” , 内橋貴之, エヌ・ティー・エス, 357-364 (2020), ISBN: 978-4-86104-781-7

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